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KMID : 0364819880260040283
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Korean Journal of Microbiology
1988 Volume.26 No. 4 p.283 ~ p.290
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Characterization of a Revertant that Restores the Export of Ribose-Binding Protein to the Periplasm in Escherichia coli
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Park, Soon-Hee/박순희
Park, Chan Kyu/Randall, Linda L./박찬규/Randall, Linda L.
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Abstract
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1
A spontaneous revertant of mutation rbsBl03 that is ribose taxis-positive was characterized. This revertant was found to be export-competent in the export of ribose-binding protein shown by the disappearance of accumulated mutant precursor protein and the export of mature ribosebinding protein to the periplasm. The reversional change was shown to be in the region of risB gene that codes for the amino terminal portion of ribose-binding protein. Analysis by high-performance liquid chromatography of peptide patterns of ribose-binding proteins confirmed the relationship between the wild-type and the revertant proteins as shown for the mutant previously (lids et aL, 19g5). When the processing rate of presursor proteins from the wild type and the revertant strain in vivo was compared by pulse-chase experiment, it was found that processing is less efficient than normal in the revertant. Purified mature proteins from both wild-type and revertant were subjected to amino acid sequencing. The results confirmed the amino acid changes deduced from the DNA sequencing and showed that processing of the revertant precursor occurred in the correct position even though there are two different amino adds present in the signal sequence.
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